ABSTRACT
Liver fibrosis, a common pathological process of most types of chronic liver diseases, is caused by the excessive deposition of extracellular matrix proteins after chronic liver injury. An increasing number of evidence has shown that oxidative stress is closely associated with the development and progression of liver fibrosis and is involved in the pathological process of liver fibrosis caused by various factors. With natural constituents and a clear structure, Chinese herbal monomers herbs have achieved a marked clinical effect in the treatment of liver fibrosis. This article reviews the research advances in monomers of Chinese herbs in the treatment of liver fibrosis by regulating oxidative stress-related signaling pathways.
ABSTRACT
Objective: To identify the chemical components in traditional Chinese herbal medicine Ginseng by high performance liquid chromatography diode array detector (HPLC-DAD) and High performance liquid chromatography-time of flight mass spectrometry (HPLC-TOF/MS). Methods: An Agilent Zorbax XDB-C18 column (250 mm X 4.6 mm, 5 μm) was used for isolation and identification of chemical components in Ginseng with a mobile phase of acetonitrile and water in gradient elution. A gradient program was used as follows: 1-35 min, 19% A; 35-55 min, 19%-29% A; 55-70 min, 29% A; 70-110 min, 29%-40% A; 110-150 min, 95% A. The flow rate was set at 1.0 ml/min, the injection volume was 20 μl, the column temperature was set at 20°C. The time-of-flight mass spectrometer was equipped with an EIS ion source. Scanning mass range was between m/z 100-1 350. Results: Thirty-nine chemical compounds in Ginseng were identified unequivocally. Conclusion: Chromatographic demonstration of 39 chemical compounds of Ginseng in one run is achieved by HPLC-TOF/MS, which provides a foundation for further study on the metabolism and action mechanism of traditional Chinese herbal medicine.
ABSTRACT
Objective: To rapidly identify the chemical components in a traditional Chinese herb Xiaochaihu decoction by high performance liquid chromatography-time of flight mass spectrometry (HPLC-TOF/MS). Methods: An Agilent ZORBAX SB-C 18 column (100 mmX3.0 mm,3.5 μm) was used for rapid separation and identification of chemical components in Xiaochaihu decoction, with a mobile phase of methanol and 0.1% formic acid aqueous solvent in gradient elution. The flow rate was 0.6 ml/min and the injection volume was 4 μl. The detector was time-of-flight mass spectrometer with an ESI ion source. Scanning mass range was m/z 200-1 500. Results: Thirty-eight chemical compounds were identified from Xiaochaihu decoction in one run. Conclusion: Thirty-eight chemical compounds have been identified in Xiaochaihu decoction by HPLC-TOF/MS in one run, which paves a way for further understanding the metabolism and mechanism of the chemical compounds in Xiaochaihu decoction.